Immunofluorescence – Principle, Types, Applications, Advantages, Disadvantages
Immunofluorescence is an antigen-antibody reaction where the antibodies are tagged (labeled) with a fluorescent dye and the antigen-antibody complex is visualized using ultra-violet (fluorescent) microscope. Fluorochrome is a dye that absorbs ultra-violet rays and emits visible light. This process is called fluorescence. Commonly used fluorochrome are Acridine Orange, Rhodamine, Lissamine, and Calcofluor white. However, these … Continue reading Immunofluorescence – Principle, Types, Applications, Advantages, Disadvantages
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