Rheumatoid Factor Test – The Complete Understanding Guide
In Rheumatoid Factor Test, we measure the amount of rheumatoid factor in our blood. Our immune system is responsible to produce rheumatoid factors which are known as the protein that…
Complement Fixation Test – Principle, Components, Procedure, Advantages, Disadvantages
In complement fixation test, Complement is used which is a biologically labile serum factor that causes the immune cytolysis i.e. lysis of antibody-coated cells which is found in normal serum.…
Western blotting – Introduction, Principle, Procedure & Uses
Introduction to Western Blotting Technique Blotting is a technique for the detection of macromolecules. There are three types of blotting methods:- Southern blotting – used in the detection of DNA…
Immunofluorescence – Principle, Types, Applications, Advantages, Disadvantages
Immunofluorescence is an antigen-antibody reaction where the antibodies are tagged (labeled) with a fluorescent dye and the antigen-antibody complex is visualized using ultra-violet (fluorescent) microscope. Fluorochrome is a dye that…
Precipitation Reaction – Mechanism, Types, & Applications
A precipitation reaction is a very important antigen-antibody reaction in which the soluble antigen reacts with its antibody in the presence of electrolytes at a suitable temperature and pH. It…
Enzyme Linked Immunosorbent Assay (ELISA)
ELISA is known as Enzyme-Linked Immunosorbent Assay because : The antigen/antibody which adsorbed on the plastic surface is known as sorbent. The antigen recognized by the antibody is known as…
Radioimmunoassay (RIA) – Principle, Procedure, Advantages, Disadvantages, & Applications
RIA stands for Radioimmunoassay. It is an immunological assay. It is basically used to determine the concentration of antigen in the blood serum of the patient with high sensitivity. It…
Agglutination Reaction: History, Mechanism, Advantages, Disadvantages, Types & Factors Influencing
Agglutination is the most common type of antigen-antibody reaction. Agglutination is the clumping or binding antigen and antibody. Agglutination reaction is the reaction in which soluble antibody interacts with particulate…
What is Serology & Serology Tests?
The morphological characteristics of plants and animals are used by descriptive natural sciences as classification criteria. However, the development of serology in the form of an independent science has made…