Complement Fixation Test – Principle, Components, Procedure, Advantages, Disadvantages

In complement fixation test, Complement is used which is a biologically labile serum factor that causes the immune cytolysis i.e. lysis of antibody-coated cells which is found in normal serum.…

Continue ReadingComplement Fixation Test – Principle, Components, Procedure, Advantages, Disadvantages

Immunofluorescence – Principle, Types, Applications, Advantages, Disadvantages

Immunofluorescence is an antigen-antibody reaction where the antibodies are tagged (labeled) with a fluorescent dye and the antigen-antibody complex is visualized using ultra-violet (fluorescent) microscope. Fluorochrome is a dye that…

Continue ReadingImmunofluorescence – Principle, Types, Applications, Advantages, Disadvantages

Radioimmunoassay (RIA) – Principle, Procedure, Advantages, Disadvantages, & Applications

RIA stands for Radioimmunoassay. It is an immunological assay. It is basically used to determine the concentration of antigen in the blood serum of the patient with high sensitivity. It…

Continue ReadingRadioimmunoassay (RIA) – Principle, Procedure, Advantages, Disadvantages, & Applications

Agglutination Reaction: History, Mechanism, Advantages, Disadvantages, Types & Factors Influencing

Agglutination is the most common type of antigen-antibody reaction. Agglutination is the clumping or binding antigen and antibody. Agglutination reaction is the reaction in which soluble antibody interacts with particulate…

Continue ReadingAgglutination Reaction: History, Mechanism, Advantages, Disadvantages, Types & Factors Influencing