Introduction The term "serology" describes the use of antigen-antibody responses in the lab for diagnosis. Serum, the liquid portion of blood where antibodies are discovered, is employed in testing, hence…
Introduction In immunology and diagnostic testing, serological cross-reactivity and serological specificity are crucial ideas. They have to do with how antibodies and antigens interact, which is essential for comprehending immune…
Introduction IgG and IgM Antibodies : Immunoglobulins (Igs), or antibodies, are glycoproteins that are created in response to an immune response and have a unique affinity for the antigens that…
In Rheumatoid Factor Test, we measure the amount of rheumatoid factor in our blood. Our immune system is responsible to produce rheumatoid factors which are known as the protein that…
In complement fixation test, Complement is used which is a biologically labile serum factor that causes the immune cytolysis i.e. lysis of antibody-coated cells which is found in normal serum.…
Introduction to Western Blotting Technique Blotting is a technique for the detection of macromolecules. There are three types of blotting methods:- Southern blotting – used in the detection of DNA…
Immunofluorescence is an antigen-antibody reaction where the antibodies are tagged (labeled) with a fluorescent dye and the antigen-antibody complex is visualized using ultra-violet (fluorescent) microscope. Fluorochrome is a dye that…
A precipitation reaction is a very important antigen-antibody reaction in which the soluble antigen reacts with its antibody in the presence of electrolytes at a suitable temperature and pH. It…
ELISA is known as Enzyme-Linked Immunosorbent Assay because : The antigen/antibody which adsorbed on the plastic surface is known as sorbent. The antigen recognized by the antibody is known as…
RIA stands for Radioimmunoassay. It is an immunological assay. It is basically used to determine the concentration of antigen in the blood serum of the patient with high sensitivity. It…